Na u sebetsa lefapheng la lipatlisiso tsa biomedical?Haeba ho joalo, mohlomong u kile ua utloela ka Sulfo-NHS.Ha karolo ea bohlokoa ea motsoako ona liphuputsong e ntse e tsoela pele ho hlokomeloa, motsoako ona o kena lilaboratoring tse ngata ho pota lefatše.Sengoliloeng sena, re tšohla hore na Sulfo-NHS ke eng le hore na hobaneng e le sesebelisoa sa bohlokoa ho ba ithutang mahlale a baeloji.
Taba ea pele, Sulfo-NHS ke eng?Lebitso le batla le le lelele, ka hona, a re le qhetsoeng.Sulfo e emetse sulfonic acid mme NHS e emetse N-hydroxysuccinimide.Ha metsoako ena e 'meli e kopana,Sulfo-NHSe hlahisoa.Motsoako ona o na le ts'ebeliso e 'maloa lipatlisisong tsa biomedical, empa e' ngoe ea thepa ea eona ea bohlokoa ke bokhoni ba ho khetha liprotheine.
Sulfo-NHS e sebetsa ka ho itšoara ka li-amine tsa mantlha (ke hore -NH2 lihlopha) ka mahlakoreng a mahlakoreng a masala a lysine liprotheine.Ha e le hantle, Sulfo-NHS e kopanya liprotheine tsa "tag", ho etsa hore ho be bonolo ho khetholla le ho hlahloba liteko tse fapaneng.Sena se entse hore libaka tse ngata tsa lipatlisiso li khone ho tsoela pele ka ho nepahala ho hoholo le lintlha tse phahameng.
Joale, Sulfo-NHS e sebelisetsoa eng?Tšebeliso e 'ngoe e tloaelehileng ea motsoako ona ke lipatlisisong tsa immunology.Sulfo-NHS e bonts'itsoe hore e tšoaea li-antibodies le li-antigen ka nepo, e bula mekhoa e mecha ea ho ithuta ka mathata le mafu a sesole sa 'mele.Ho feta moo,Sulfo-NHSe ka sebelisoa lithutong tsa ho sebelisana ha protheine le protheine kaha e lumella bafuputsi ho lemoha kapele le ha bonolo ha liprotheine tse peli li kopana.
Sebaka se seng seo Sulfo-NHS e sebelisoang haholo ke sa proteomics.Proteomics e ithuta sebopeho le tšebetso ea liprotheine tsohle tse phelang, leSulfo-NHSke sesebelisoa sa bohlokoa tlhahlobong ena.Ka ho tšoaea liprotheine ka Sulfo-NHS, bafuputsi ba ka etsa liteko ho fumana lintlha tse qaqileng haholoanyane mabapi le proteome ea 'mele e fanoeng, e ka thusang ho tseba li-biomarker tse ka bang teng bakeng sa lefu.
Sulfo-NHS e boetse e na le seabo ntlafatsong ea meriana e mecha.Ha bafuputsi ba leka ho hlahisa moriana o mocha, ho bohlokoa ho etsa bonnete ba hore o shebane le protheine e reriloeng eseng protheine efe kapa efe 'meleng.Ka ho sebelisaSulfo-NHSho khetha liprotheine, bafuputsi ba ka tseba sepheo se nepahetseng sa lithethefatsi tse ka bang teng, tse ka thusang ho potlakisa ts'ebetso ea nts'etsopele ea lithethefatsi.
Kahoo u na le eona!Sulfo-NHS e ka 'na ea se ke ea e-ba lentsoe le tsebahalang ka ntle ho sechaba sa saense, empa motsoako ona o potlakela ho fetoha sesebelisoa sa bohlokoa lipatlisisong tsa biomedical.Ho tloha lipatlisisong tsa immunology ho ea ho proteomics ho nts'etsopele ea lithethefatsi, Sulfo-NHS e thusa bafuputsi ho etsa tsoelo-pele e kholo libakeng tsena 'me re thabile ho bona hore na ke lintho life tse sibolotsoeng tse tlang ka mor'a moo.
Nako ea poso: Jun-12-2023